-
1.
Comparison of the Effects of Eight Different Topical Non-Steroidal Anti-Inflammatory Drugs on Reducing Intravitreal Injection-induced Pain.
Sakallioğlu, AK, Kaya, S, Garip, R, Güçlü, H
Retina (Philadelphia, Pa.). 2024
Abstract
PURPOSE To compare topical nonsteroidal anti-inflammatory drug (NSAID) efficacy on intravitreal injection (IVI)-induced pain reduction, and determine the most efficient topical NSAID. METHODS This randomized controlled study included 662 eyes of 662 patients. Based on the types of NSAID administered before IVI, eight subgroups were formed. In the control group, a sterile saline solution was applied instead of NSAIDs. The visual analog scale (VAS) was used to assess pain scores after IVI. The VAS scores were noted immediately and 6 hours following injection (6th hour). RESULTS Nepafenac 0.3%, 0.1%, and bromfenac had the lowest scores, immediately after and after 6 hours, with no significant differences. Diclofenac and ketorolac had higher VAS scores than the first trio but lower scores than the control group. Flurbiprofen, pranoprofen, and indomethacin did not significantly affect immediate pain; however, at the 6th hour, the VAS scores were significantly reduced. CONCLUSION Nepafenac 0.3%, 0.1%, and bromfenac were the most effective NSAIDs for pain reduction. Although some NSAIDs did not have a significant effect on immediate pain, they all provided significant benefit at the 6th hour.
-
2.
MediNet: transfer learning approach with MediNet medical visual database.
Reis, HC, Turk, V, Khoshelham, K, Kaya, S
Multimedia tools and applications. 2023;:1-44
Abstract
The rapid development of machine learning has increased interest in the use of deep learning methods in medical research. Deep learning in the medical field is used in disease detection and classification problems in the clinical decision-making process. Large amounts of labeled datasets are often required to train deep neural networks; however, in the medical field, the lack of a sufficient number of images in datasets and the difficulties encountered during data collection are among the main problems. In this study, we propose MediNet, a new 10-class visual dataset consisting of Rontgen (X-ray), Computed Tomography (CT), Magnetic Resonance Imaging (MRI), Ultrasound, and Histopathological images such as calcaneal normal, calcaneal tumor, colon benign colon adenocarcinoma, brain normal, brain tumor, breast benign, breast malignant, chest normal, chest pneumonia. AlexNet, VGG19-BN, Inception V3, DenseNet 121, ResNet 101, EfficientNet B0, Nested-LSTM + CNN, and proposed RdiNet deep learning algorithms are used in the transfer learning for pre-training and classification application. Transfer learning aims to apply previously learned knowledge in a new task. Seven algorithms were trained with the MediNet dataset, and the models obtained from these algorithms, namely feature vectors, were recorded. Pre-training models were used for classification studies on chest X-ray images, diabetic retinopathy, and Covid-19 datasets with the transfer learning technique. In performance measurement, an accuracy of 94.84% was obtained in the traditional classification study for the InceptionV3 model in the classification study performed on the Chest X-Ray Images dataset, and the accuracy was increased 98.71% after the transfer learning technique was applied. In the Covid-19 dataset, the classification success of the DenseNet121 model before pre-trained was 88%, while the performance after the transfer application with MediNet was 92%. In the Diabetic retinopathy dataset, the classification success of the Nested-LSTM + CNN model before pre-trained was 79.35%, while the classification success was 81.52% after the transfer application with MediNet. The comparison of results obtained from experimental studies observed that the proposed method produced more successful results.
-
3.
An insight on medicinal attributes of 1,2,3- and 1,2,4-triazole derivatives as alpha-amylase and alpha-glucosidase inhibitors.
Sharma, A, Dubey, R, Bhupal, R, Patel, P, Verma, SK, Kaya, S, Asati, V
Molecular diversity. 2023
Abstract
Diabetes Mellitus (DM) is the globe's common leading disease which is caused by high consumption of glucose. DM compiles groups of metabolic disorders which are characterized by inadequate secretion of insulin from pancreas, resulting in hyperglycemia condition. Many enzymes play a vital role in the metabolism of carbohydrate known as α-amylase and α-glucosidase which is calcium metalloenzyme that leads to breakdown of complex polysaccharides into glucose. To tackle this problem, search for newer antidiabetic drugs is the utmost need for the treatment and/or management of increasing diabetic burden. The inhibition of α-amylase and α-glucosidase is one of the effective therapeutic approaches for the development of antidiabetic therapeutics. The exhaustive literature survey has shown the importance of medicinally privileged triazole specifically 1,2,3-triazol and 1,2,4-triazoles scaffold tethered, fused and/or clubbed with other heterocyclic rings structures as promising agents for designing and development of novel antidiabetic therapeutics. Molecular hybrids namely pyridazine-triazole, pyrazoline-triazole, benzothiazole-triazole, benzimidazole-triazole, curcumin-triazole, (bis)coumarin-triazole, acridine-9-carboxamide linked triazole, quinazolinone-triazole, xanthone-triazole, thiazolo-triazole, thiosemicarbazide-triazole, and indole clubbed-triazole are few examples which have shown promising antidiabetic activity by inhibiting α-amylase and/or α-glucosidase. The present review summarizes the structure-activity relationship (SAR), enzyme inhibitory activity including IC50 values, percentage inhibition, kinetic studies, molecular docking studies, and patents filed of the both scaffolds as alpha-amylase and alpha-glucosidase inhibitors, which may be used for further development of potent inhibitors against both enzymes.
-
4.
A comprehensive and current review on the role of flavonoids in lung cancer-Experimental and theoretical approaches.
Berk, Ş, Kaya, S, Akkol, EK, Bardakçı, H
Phytomedicine : international journal of phytotherapy and phytopharmacology. 2022;:153938
Abstract
BACKGROUND It is well-known that flavonoids, which can be easily obtained from many fruits and vegetables are widely preferred in the treatment of some important diseases. Some researchers noted that these chemical compounds exhibit high inhibition effect against various cancer types. Many experimental studies proving this ability of the flavonoids with high antioxidant activity are available in the literature. PUROPOSE The main aim of this review is to summarize comprehensively anticancer properties of flavonoids against the lung cancer in the light of experimental studies and well-known theory and electronic structure principles. In this review article, more detailed and current information about the using of flavonoids in the treatment of lung cancer is presented considering theoretical and experimental approaches. STUDY DESIGN In addition to experimental studies including the anticancer effects of flavonoids, we emphasized the requirement of the well-known electronic structure principle in the development of anticancer drugs. For this aim, Conceptual Density Functional Theory should be considered as a powerful tool. Searching the databases including ScienceDirect, PubMed and Web of Science, the suitable reference papers for this project were selected. METHODS Theoretical tools like DFT and Molecular Docking provides important clues about anticancer behavior and drug properties of molecular systems. Conceptual Density Functional Theory and CDFT based electronic structure principles and rules like Hard and Soft Acid-Base Principle (HSAB), Maximum Hardness Principle, Minimum Polarizability, Minimum Electrophilicity Principles and Maximum Composite Hardness Rule introduced by one of the authors of this review are so useful to predict the mechanisms and powers of chemical systems. Especially, it cannot be ignored the success of HSAB Principle in the explanations and highlighting of biochemical interactions. RESULTS Both theoretical analysis and experimental studies confirmed that flavonoids have higher inhibition effect against lung cancer. In addition to many superior properties like anticancer activity, antimicrobial activity, antioxidant activity, antidiabetic effect of flavonoids, their toxicities are also explained with the help of published popular papers. Action modes of the mentioned compounds are given in detail. CONCLUSION The review includes detailed information about the mentioned electronic structure principles and rules and their applications in the cancer research. In addition, the epidemiology and types of lung cancer anticancer activity of flavonoids in lung cancer are explained in details.
-
5.
Evaluation of the effects of fear and anxiety on nutrition during the COVID-19 pandemic in Turkey.
Kaya, S, Uzdil, Z, Cakiroğlu, FP
Public health nutrition. 2021;24(2):282-289
-
-
-
Free full text
-
Plain language summary
The current treatment for COVID-19 is mainly focused on infection control and effective vaccine therapy because of extensive measures to reduce person-to-person transmission. However, the psychosocial repercussions of the COVID-19 pandemic have not yet been fully considered. The aim of this study was to evaluate the nutritional attitudes and habits that changed during the COVID-19 pandemic due to fear and anxiety status. The study included 1012 individuals (n=827 female, n=185 male) living in Turkey with an age range between 18 and 65 years. Results indicate: - that 26·7 % of the participants used nutritional supplements in particular vitamin C, vitamin D, vitamin B12 and zinc. - an inverse association between anxiety, fear and nutrition habits during the COVID-19 pandemic. - differences in shopping preferences, processes applied to foods and vegetables and fruits due to the fear of virus contamination from the surfaces. Authors conclude that continuous surveillance of the psychological consequences for outbreaks should become routine as part of preparedness efforts worldwide.
Abstract
OBJECTIVE The aim of this study was to evaluate effects of fear and anxiety on nutrition during the COVID-19 pandemic. DESIGN Participants were recruited by an online survey in this cross-sectional study. The questionnaire included general demographic characteristics, level of fear and anxiety, and nutritional habits. The Fear of COVID-19 Scale (FCV-19S) and Generalized Anxiety Disorder-7 test (GAD-7) were used to determine fear and anxiety. SETTING Turkey. PARTICIPANTS A total sample consisted of 1012 adults. RESULTS In pandemic, fear and anxiety caused individuals to skip breakfast and snacks less, but more at lunch. A positive significant correlation was observed between the increased consumption of yoghurt, cheese and water and FCV-19S scores. There was a positive significant correlation between cheese, legume, nuts-seeds, cake-cookies, dessert and tea consumption and GAD-7 scores. A 1-unit increase in FCV-19S scores affected 1·04 times of increased consumption of yoghurt, kefir, cheese, nuts-seeds, fruit (dry) and rice-pasta. A 1-unit increase in GAD-7 scores affected 1·03 times of increased consumption of egg and fruit (fresh); 1·04 times of increased consumption of cheese and other vegetables; 1·05 times of increased consumption of milk, meat, poultry, fish, legume, nuts-seeds, fruit (dry), cake-cookies and tea; 1·07 times of increased consumption of rice-pasta and coffee and 1·08 times of increased consumption of bread and dessert. CONCLUSIONS In pandemic, anxiety and fear led to changes in individuals' nutritional habits and food preferences. Continuous surveillance of psychological consequences for outbreaks should become routine as part of preparedness efforts worldwide. In addition, the effects of these psychological problems on nutrition should be evaluated.
-
6.
Mesoporous silica-based bioactive glasses for antibiotic-free antibacterial applications.
Kaya, S, Cresswell, M, Boccaccini, AR
Materials science & engineering. C, Materials for biological applications. 2018;:99-107
Abstract
Bioactive glasses (BGs) are being used in several biomedical applications, one of them being as antibacterial materials. BGs can be produced via melt-quenching technique or sol-gel method. Bactericidal silver-doped sol-gel derived mesoporous silica-based bioactive glasses were reported for the first time in 2000, having the composition 76SiO2-19CaO-2P2O5-3Ag2O (wt%) and a mean pore diameter of 28nm. This review paper discusses studies carried out exploring the potential antibacterial applications of drug-free mesoporous silica-based BGs. Bioactive glasses doped with metallic elements such as silver, copper, zinc, cerium and gallium are the point of interest of this review, in which SiO2, SiO2-CaO and SiO2-CaO-P2O5 systems are included as the parent glass compositions. Key findings are that silica-based mesoporous BGs offer a potential alternative to the systemic delivery of antibiotics for prevention against infections. The composition dependent dissolution rate and the concentration of the doped elements affect the antibacterial efficacy of BGs. A balance between antibacterial activity and biocompatibility is required, since a high dose of metallic ion addition can cause cytotoxicity. Typical applications of mesoporous BGs doped with antibacterial ions include bone tissue regeneration, multifunctional ceramic coatings for orthopedic devices and orbital implants, scaffolds with enhanced angiogenesis potential, osteostimulation and antibacterial properties for the treatment of large bone defects as well as in wound healing.
-
7.
A Controlled, Randomized Double-Blind Study to Evaluate the Safety and Efficacy of Chitosan-N-Acetylcysteine for the Treatment of Dry Eye Syndrome.
Schmidl, D, Werkmeister, R, Kaya, S, Unterhuber, A, Witkowska, KJ, Baumgartner, R, Höller, S, O'Rourke, M, Peterson, W, Wolter, A, et al
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics. 2017;(5):375-382
Abstract
PURPOSE This study was designed to evaluate the effect of chitosan-N-acetylcysteine (C-NAC) eye drops on tear film thickness (TFT) in patients with dry eye syndrome (DES). METHODS This was a controlled, randomized, double-blind clinical investigation with patients assigned to 2 cohorts. In Cohort I, 21 patients were randomized to receive 1 instillation of C-NAC eye drops in 1 eye and placebo (normal saline solution) in the contralateral eye. In Cohort II, 17 patients were randomized to receive C-NAC eye drops once (QD) or twice (BID) daily for 5 days. TFT was assessed with a custom-built ultrahigh-resolution optical coherence tomography system. RESULTS In Cohort I, mean TFT increased from 3.9 ± 0.5 μm predose to 4.8 ± 1.1 μm 10 min postdose after treatment with C-NAC. The increase was significantly different from placebo over time (P < 0.0001) and remained stable until 24 h postdose. In Cohort II, TFT increased with QD and BID instillation, with no significant difference between regimens. In both groups, Ocular Surface Disease Index scores improved, fewer patients presented with corneal damage, and symptoms of ocular discomfort/conjunctival redness were reduced. CONCLUSIONS A single instillation of C-NAC significantly increased mean TFT in patients with DES as early as 10 min after instillation and lasted for 24 h. The magnitude of the increase in TFT following a single instillation was comparable with that after instillation twice daily over 5 days. Corneal damage improved in >60% of patients. C-NAC could be a viable treatment option for DES.
-
8.
Integrase Strand Transfer Inhibitors (INSTIs) Resistance Mutations in HIV-1 Infected Turkish Patients.
Sayan, M, Gündüz, A, Ersöz, G, İnan, A, Deveci, A, Özgür, G, Sargın, F, Karagöz, G, İnci, A, İnan, D, et al
HIV clinical trials. 2016;(3):109-13
Abstract
OBJECTIVES Integrase strand transfer inhibitor (INSTI) is a new class of antiretroviral (ARV) drugs designed to block the action of the integrase viral enzyme, which is responsible for insertation of the HIV-1 genome into the host DNA. The aim of this study was to evaluate for the first time INSTI resistance mutations in Turkish patients. METHODS This study was conducted in Turkey, between April 2013 and April 2015 using 169 HIV-1-infected patients (78 ARV naive patients and 91 ARV-experienced patients). Laboratory and clinical characteristics of ARV naive and ARV-experienced patients were as follows: gender (M/F): 71/7 and 80/11, median age: 38 and 38.4; median CD4(+) T-cell: 236 and 216 cells/mm(3), median HIV-1 RNA: 4.95+E5 and 1.08E+6 copies/ml. Population-based seqeunces of the reverse transcriptase, protease, and integrase domains of the HIV-1 pol gene were used to detect HIV-1 drug resistance mutations. RESULT INSTI resistance mutations were not found in recently diagnosed HIV-1-infected patients. However, ARV-experienced patients had major resistance mutations associated with raltegravir and elvitegravir; the following results were generated:F121Y, Y143R, Q148R and E157Q (6/91 - 6.6%). CONCLUSIONS The prevalence of INSTI resistant mutations in ART-experienced patients suggested that resistance testing must be incorporated as an integral part of HIV management with INSTI therapies.
-
9.
Tear film thickness after treatment with artificial tears in patients with moderate dry eye disease.
Schmidl, D, Schmetterer, L, Witkowska, KJ, Unterhuber, A, dos Santos, VA, Kaya, S, Nepp, J, Baar, C, Rosner, P, Werkmeister, RM, et al
Cornea. 2015;(4):421-6
Abstract
PURPOSE This study was designed to investigate the effect of a single-drop instillation of different lacrimal substitutes on tear film thickness (TFT) assessed with optical coherence tomography in patients with mild to moderate dry eye disease. METHODS The study was performed in a randomized, double-masked, controlled parallel group design. Patients received a single dose of either unpreserved trehalose 30 mg/mL and sodium hyaluronate 1.5 mg/mL (TH-SH, Thealoz Duo), unpreserved sodium hyaluronate, 0.15% (HA, Hyabak) or sodium chloride, 0.9% (NaCl, Hydrabak) eye drops. Sixty patients finished the study according to the protocol. TFT was measured with a custom-built ultrahigh-resolution Fourier domain optical coherence tomography system providing a resolution of 1.2 μm. RESULTS The mean TFT before treatment was 2.5 ± 0.4 μm. Ten minutes after instillation, TFT significantly increased in the TH-SH group from 2.4 ± 0.4 to 3.1 ± 0.9 μm (P < 0.01) and in the HA group from 2.4 ± 0.3 to 2.9 ± 0.5 μm (P < 0.01), whereas no significant change was observed in the NaCl group (from 2.6 ± 0.4 to 2.7 ± 0.4 μm, P = 0.76). The increase in TFT remained statistically significant up to 240 minutes after administration of TH-SH. In contrast, the increase in TFT after administration of HA was only statistically significant at 10, 20, and 40 minutes after drop instillation. CONCLUSIONS The findings of this study indicate that single instillation of TH-SH and HA eye drops increases TFT in patients with dry eye disease. The data also indicate longer corneal residence of the TH-containing eye drops. The effect of multiple instillation and long-term use of artificial tears on TFT warrants further investigation.
-
10.
Neurovascular dysfunction precedes neural dysfunction in the retina of patients with type 1 diabetes.
Lasta, M, Pemp, B, Schmidl, D, Boltz, A, Kaya, S, Palkovits, S, Werkmeister, R, Howorka, K, Popa-Cherecheanu, A, Garhöfer, G, et al
Investigative ophthalmology & visual science. 2013;(1):842-7
Abstract
PURPOSE A variety of studies have shown that flicker-induced vasodilatation is reduced in patients with diabetes. It is, however, unclear whether reduced neural activity or abnormal neurovascular coupling is the reason for this phenomenon. In the present study, we hypothesized that retinal neurovascular dysfunction precedes neural dysfunction in patients with early type 1 diabetes. METHODS In the present study, 50 patients with type 1 diabetes without retinopathy and 50 healthy age- and sex-matched control subjects were included. The retinal vascular response to flicker stimulation was measured using the dynamic Retinal Vessel Analyzer. In addition, the response in retinal blood velocity to flicker stimulation as assessed with laser Doppler velocimetry was studied in a subgroup of patients. Pattern electroretinography (ERG) was used to measure neural retinal function. RESULTS The flicker responses of both retinal arteries and veins were significantly reduced in patients with diabetes (veins in the diabetic group: 3.5 ± 2.3% versus healthy control group: 4.6 ± 2.0%; P = 0.022 between groups, whereas arteries in the diabetic group: 2.0 ± 2.7% versus healthy control group: 3.8 ± 1.7%; P < 0.001 between groups). Likewise, the response of retinal blood velocity was reduced in patients with diabetes, although adequate readings could only be obtained in a subgroup of subjects (diabetic group [n = 22]: 19 ± 7%; healthy control group [n = 24]: 43 ± 19% P < 0.001 between groups). The parameters of pattern ERG were not different between the two groups. CONCLUSIONS The study confirms that flicker responses are reduced early in patients with type 1 diabetes. This is seen before alterations in pattern ERG indicating abnormal neurovascular coupling.